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目的:比较分析新疆哈萨克民族食管癌淋巴结转移患者和健康人血清蛋白质谱的差异,建立新疆哈萨克民族食管癌淋巴结转移的蛋白质指纹诊断模型,并筛选与食管癌淋巴结转移的相关蛋白质。方法:采用弱阳离子交换蛋白芯片(CM10)和表面加强激光解吸离子化飞行时间质谱技术,检测分析了21例食管癌有淋巴结转移,20例食管癌无淋巴结转移的患者和20例正常对照的哈萨克民族血清,用支持向量机方法建立蛋白质指纹图谱诊断模型,用留一法交叉验证作为评估模型判别效果的方法。两组之间的蛋白质峰的比较采用Wilconxon秩和检验。结果:结果显示新疆哈萨克民族食管癌淋巴结转移患者和健康人血清的蛋白质荷比峰值图谱明显不同。比较21例淋巴结阳性患者组与20例淋巴结阴性组食管癌血清蛋白质指纹图谱数据,其中5个峰差异有统计学意义(P<0.05)。3个峰在食管癌淋巴结阴性组呈高表达,2个峰呈低表达。结论:建立的诊断模型在区分哈萨克民族食管癌淋巴结转移患者上,具有较高的灵敏度和特异度;筛选出的蛋白标记物可能为新疆哈萨克民族食管癌淋巴结转移提供依据。
OBJECTIVE: To compare and analyze the differences of serum protein profiles between Kazakh patients with esophageal cancer lymph node metastasis and healthy subjects in Xinjiang, and to establish a protein fingerprinting diagnostic model of lymph node metastasis in Kazakh ethnic esophageal cancer in Xinjiang and to screen out the related proteins in lymph node metastasis of esophageal cancer. Methods: 21 cases of esophageal cancer with lymph node metastasis, 20 cases of esophageal cancer without lymph node metastasis and 20 cases of normal control were studied by using weak cation exchange protein chip (CM10) and surface enhanced laser desorption / ionization time of flight mass spectrometry National serum, using the method of support vector machine to establish the diagnostic model of protein fingerprinting, and using the method of cross-validation as a method to evaluate the discriminant effect of the model. Comparison of protein peaks between the two groups was performed using the Wilcoxon rank sum test. Results: The results showed that the protein peak-to-peak ratio of serum in patients with lymph node metastasis of Kazakh ethnic esophageal cancer in Xinjiang was significantly different from those in healthy people. Serum protein fingerprinting data of 21 patients with positive lymph node group and 20 patients with negative node group were compared. There were significant differences among the 5 peaks (P <0.05). Three peaks were highly expressed in lymph node-negative group of esophageal cancer, and two peaks were low expression. Conclusion: The established diagnostic model has higher sensitivity and specificity in differentiating patients with lymph node metastasis from Kazakh ethnic esophageal cancer. The screened protein markers may provide the basis for lymph node metastasis in Kazakh ethnic esophageal cancer in Xinjiang.