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目的:建立测定新西兰兔血浆中鹿角缩酮B的反相高效液相色谱方法,并测定静脉给药后新西兰兔血浆中鹿角缩酮B浓度及药动学参数。方法:血浆样品经酸化后用乙酸乙酯进行液液萃取,化合物1为内标,色谱柱为Phenomenex C18,流动相为甲醇-乙腈-水(30∶30∶40),流速0.8 mL.min-1,检测波长220 nm。结果:本方法中内源性物质不干扰测定,鹿角缩酮B在0.4～50μg.mL-1范围内线性良好(r=0.9995),检测限为0.2μg.mL-1;高中低浓度平均回收率为85.2%～105.5%,日内和日间精密度RSD均小于10%。结论:首次建立了鹿角缩酮B在新西兰兔体内的分析方法,为鹿角缩酮B的体内分析奠定了方法学基础。本方法专属、准确、灵敏,适用于测定新西兰兔的血药浓度;静脉给药后鹿角缩酮B在体内药代动力学符合二室模型。
Objective: To establish an RP-HPLC method for the determination of anthrone B in New Zealand rabbits plasma and to determine the concentration and pharmacokinetic parameters of anticorne ketal B in New Zealand rabbit plasma after intravenous administration. Methods: The plasma samples were acidified and extracted with ethyl acetate. The internal standard of compound 1 was Phenomenex C18 with the mobile phase of methanol-acetonitrile-water (30:30:40) at a flow rate of 0.8 mL · min- 1, detection wavelength 220 nm. Results: In this method, the endogenous substances did not interfere with the determination. The antler ketal B had a good linearity (r = 0.9995) in the range of 0.4-50 μg.mL-1 with a detection limit of 0.2 μg.mL-1. Rates ranged from 85.2% to 105.5% with intraday and intraday RSDs less than 10%. CONCLUSION: The method for the determination of antler ketal B in New Zealand rabbits was established for the first time, which laid the foundation of methodology for the in vivo analysis of antler ketal B in vivo. The method is specific, accurate and sensitive, and is suitable for determining the blood concentration of New Zealand rabbits. The pharmacokinetics of antler Ketone B after intravenous administration conforms to the two-compartment model.