论文部分内容阅读
为验证已建立的水培苗直接浇菌法的准确性与适用范围,利用此方法分析了5个致病相关基因敲除突变体对巴西蕉的致病力,评价了巴西蕉、皇帝蕉、粉蕉、宝岛蕉4个香蕉品种对4株尖镰孢菌古巴专化型病原菌株的抗性,并观察了接菌后巴西蕉根部与球茎组织的病原菌入侵过程。结果表明,foste12基因敲除突变株△Foste12菌株对巴西蕉的致病力与野生型菌株相比下降41%,且与传统的盆栽苗伤根淋菌法测试的下降趋势基本一致;皇帝蕉、宝岛蕉对4株病原菌均表现为中抗或高抗,粉蕉均表现为感病或高感;且观察到病原菌孢子附着根部、菌丝入侵根细胞间隙、维管束组织内的菌丝纵向生长及球茎组织内的菌丝侵入定殖等入侵过程。研究证实水培苗直接浇菌法可用于病原菌致病机理分析及与不同香蕉种质资源对尖镰孢菌古巴专化型的室内抗性评价。
In order to verify the accuracy and applicability of the direct watering method for establishing hydroponics seedlings, the pathogenicity of five disease-associated gene knockout mutants on Brazilian banana was analyzed by this method. The effects of Brazilian banana, Four banana cultivars were used to test the resistance of four banana cultivars to Fusarium oxysporum f.sp. virulent and the pathogen invasion process was observed after the inoculation. The results showed that the foste12 knockout mutant strain △ Foste12 showed a 41% decrease in virulence compared with the wild-type strain, and the decline trend was basically the same as that of the traditional gonococcal vaccine test. Island banana on the four pathogens showed resistance or high resistance, were banana showed susceptibility or high flu; and observed pathogen spores attached to the root, hyphae invasion of the root cell gap, vascular bundle within the longitudinal growth of mycelium And mycelium invagination colonization and other invasive processes. Studies have confirmed that direct watering seedling culture method can be used for pathogenic bacteria pathogenicity analysis and different banana germplasm resources on the Cephalosporins Cuban specialized type of indoor resistance evaluation.