论文部分内容阅读
目的研究促黄体激素释放激素(LHRH)基因与绿脓杆菌外毒素A(PE40)基因重组蛋白(LHRH-PE40)对非小细胞肺癌细胞A549的毒性作用。方法从用扫描电镜观察LHRH-PE40对A549细胞产生的细胞毒性作用,用流式细胞仪检测LHRH-PE40对A549细胞周期的影响。结果扫描电镜观察可见LHRH-PE40对A549细胞产生的毒性作用使A549细胞表面凸凹不平,流式细胞仪检测发现LHRH-PE40使A549细胞出现明显的凋亡。结论 LHRH-PE40可以诱导A549细胞凋亡。
Objective To investigate the toxic effects of the luteinizing hormone-releasing hormone (LHRH) gene and the Pseudomonas exotoxin A (PE40) gene recombinant protein (LHRH-PE40) on non-small cell lung cancer cell line A549. Methods The cytotoxic effect of LHRH-PE40 on A549 cells was observed by scanning electron microscope. The effect of LHRH-PE40 on the cell cycle of A549 cells was detected by flow cytometry. RESULTS: Scanning electron microscopy showed that the cytotoxic effect of LHRH-PE40 on A549 cells made the surface of A549 cells uneven. Flow cytometry showed that LHRH-PE40 caused obvious apoptosis in A549 cells. Conclusion LHRH-PE40 can induce apoptosis in A549 cells.