论文部分内容阅读
目的:探讨钙调神经磷酸酶（calcineurin,CaN）依赖的信号通路在血管紧张素Ⅱ（Ang Ⅱ）刺激的大鼠血管平滑肌细胞（VSMCs）增殖中的作用。方法:采用组织贴块法,体外原代培养大鼠胸主动脉平滑肌细胞;Ang Ⅱ刺激培养的大鼠 VSMCs增殖;CaN特异性抑制剂环孢素 A（CsA）阻断Ang Ⅱ刺激的大鼠VSMCs中CaN依赖的信号转导通路;观察各因素对CaN活性、细胞增殖活度、增殖细胞核抗原（PCNA）表达水平的影响。结果:在一定范围内,Ang Ⅱ（10-8～10-6mol/L）以浓度依赖性方式增加VSMCs的CaN活性,同时细胞增殖活度（AMTT值）增高,与对照组相比,差异有统计学意义（P<0.01或P<0.001）。CsA（1 μmol/L）抑制CaN活性后,明显降低Ang Ⅱ（10-7mol/L）刺激的VSMCs增殖活度及核内PCNA的表达水平（OD值）,与Ang Ⅱ组比较,差异显著（P<0.001）。结论:CaN依赖的信号通路在Ang Ⅱ刺激的血管平滑肌细胞增殖中发挥重要作用。
AIM: To investigate the role of calcineurin (CaN) -dependent signaling pathway in the proliferation of rat vascular smooth muscle cells (VSMCs) stimulated with angiotensin Ⅱ (Ang Ⅱ). Methods: Rat thoracic aortic smooth muscle cells were primarily cultured in vitro by means of tissue block method. The cultured rat VSMCs were stimulated with Ang Ⅱ, and the Ca (superscript 2 +) - specific inhibitor CsA blocked Ang Ⅱ-stimulated rats CaN-dependent signal transduction pathway in VSMCs. The effects of various factors on the activity of CaN, the activity of cell proliferation and the expression of proliferating cell nuclear antigen (PCNA) were observed. Results: Within a certain range, Ang Ⅱ (10-8 ~ 10-6mol / L) increased the CaN activity of VSMCs in a concentration-dependent manner, meanwhile, the cell proliferation activity (AMTT) increased. Compared with the control group, Statistical significance (P <0.01 or P <0.001). The inhibitory effect of CsA (1 μmol / L) on the proliferation of VSMCs stimulated by Ang Ⅱ (10-7 mol / L) and the expression of PCNA in the nucleus after the inhibition of CaN activity were significantly different from those in Ang Ⅱ group P <0.001). Conclusion: CaN-dependent signaling pathway plays an important role in the proliferation of vascular smooth muscle cells stimulated by Ang Ⅱ.