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对２例正常人、３例骨髓增生异常综合征、２例慢性再生障碍性贫血、５例慢性髓细胞白血病病人骨髓细胞体外培养显示，各种骨髓细胞对造血生长因子ｒｈＧＭＣＳＦ、ｒｈＥＰＯ、ｒｈγＩＦＮ、－γｈＩＬ－３联合作用的反应较为一致，只是反应程度不同，ＧＭ－ＣＳＦ、ＲＰＯ双因子联合应用时对ＣＦＵ－ＧＭ形成的作用与ＧＭ－ＣＳＦ单因子作用相似。而当培养体系中同时加入ＩＬ－３，ＧＭ－ＣＳＦ、ＥＰＯ时，表现出明显的协同与叠加作用，各标本中集（丛）落数量和细胞数均显著增加，红系粒系培养血中都可见到较多混合性集落。γＩＦＮ抑制ＣＦＵ－ＧＭ，ＢＦＵ－Ｅ形成，而当与ＩＬ－３．ＧＭ－ＣＳＦ、ＥＰＯ联合应用时，其抑制克隆形成的作用被抵消，在正常人及２例ＭＤＳ标本中，ＢＦＵ－Ｅ、ＣＦＵ－ＧＭ克隆生长旺盛，似乎表现出协同作用．
In vitro culture of bone marrow cells from 2 normal subjects, 3 myelodysplastic syndromes, 2 chronic aplastic anemia patients and 5 chronic myeloid leukemia patients showed that allogenic myeloid leukemia cells were significantly inhibited by hematopoietic growth factors rhGMCSF, rhEPO, rhγIFN, The response of γhIL-3 was consistent, but with different degrees of response. The combined effect of GM-CSF and RPO on CFU-GM was similar to that of GM-CSF alone. When IL-3, GM-CSF and EPO were simultaneously added into the culture system, synergism and superposition were observed. The number of clusters and the number of cells in each sample were significantly increased Can see more mixed colonies. γIFN inhibits the formation of CFU-GM, BFU-E, and when combined with IL-3. GM-CSF and EPO, the inhibition of clonogenicity was offset. BFU-E and CFU-GM clones grew well and appeared synergistic in normal subjects and two MDS specimens.