论文部分内容阅读
目的探讨吸入视黄酸(RA)对急性发作期和慢性持续期哮喘大鼠多种炎症因子影响和作用机制。方法以鸡卵清蛋白(OVA)激发大鼠7d或30 d以制备急性或慢性哮喘模型。在1-7 d或23-30 d期间分别用10μg/ml RA(RA组)、10μg RA+5μg/ml budesonide(RA+BU组)、石蜡油(PO组)雾化吸入治疗1周。急性模型7 d后取血清与肺脏进行检测,慢性模型30 d及随后休息10 d取上清与肺脏进行检测。用免疫荧光、ELISA法检查肺间质细胞因子与血Th因子表达。结果急性发作期PO组胸腺基质淋巴细胞生成素(TSLP)、核因子-κB(NF-κB)、干细胞因子(SCF)表达增加。RA能下调TSLP,但SCF明显上调。慢性持续期PO组TSLP、NF-κB、SCF、IL-4随时间逐步上调,肺感染病灶多,肺泡隔中度增宽。RA组主要炎症因子水平逐渐降低,其中TSLP、IL-4、SCF减少具统计学差异;肺感染病灶少,肺泡隔增宽较轻。BU+RA组下调TSLP表达,但SCF持续上调,肺泡出血与感染较重。结论 RA通过活化巨噬细胞,短暂加重急性发作期哮喘过敏反应,但抑制慢性持续期哮喘肺间质过敏炎症,具免疫调整和抗感染作用。
Objective To investigate the effect and mechanism of inhaled retinoic acid (RA) on inflammatory cytokines in acute and chronic asthmatic rats. Methods Rats were challenged with chicken ovalbumin (OVA) for 7 days or 30 days to prepare acute or chronic asthma models. The rats were treated with nebulization of 10μg / ml RA (RA group), 10μg RA + 5μg / ml budesonide (RA + BU group) and paraffin oil (PO group) for 1 week respectively during 1-7 days or 23-30 days. After 7 days of acute model, serum and lung were taken for detection. After 30 days of chronic model and 10 days after resting, supernatant and lung were taken for detection. Immunofluorescence and ELISA were used to detect the expression of interstitial cytokines and blood Th factors. Results The expression of TSLP, NF-κB and SCF increased in PO group during acute exacerbation. RA can down-regulate TSLP, but SCF is significantly up-regulated. TSLP, NF-κB, SCF and IL-4 in chronic persistent PO group were gradually increased over time, with more lung infections and more moderate alveolar septum. The levels of major inflammatory cytokines decreased gradually in RA group, of which the difference of TSLP, IL-4 and SCF was statistically significant; BU + RA group down-regulated TSLP expression, but SCF continued to increase, with severe alveolar hemorrhage and infection. Conclusion RA activates macrophages to exacerbate the allergic reaction of asthma in acute episode, but inhibits the chronic interstitial allergic inflammation in asthma with immune response and immune response.