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为了研究接种S.fredii WGF03及其exo D基因突变体对大豆结瘤及土壤的微生物群落影响,进一步了解exo D基因的功能,在大豆盛花期摘取每株大豆的根瘤并计数,利用PCR-DGGE电泳结合测序技术分析土壤的微生物群落。结果表明,大豆接种S.fredii WGF03后,根瘤数比不接种组增加191.67%。而大豆接种驻exo D突变体的根瘤数最少,比不接种组减少了16.67%。与空白相比,种植大豆后土壤细菌的种类和数量明显增加;接种不同根瘤菌后,细菌种类及细菌多样性也有变化;测序结果显示,土壤中细菌以Proteobacteria为主,占45.5%,土壤中土著根瘤菌为Bradyrhizobium。总之,S.fredii WGF03能够促进大豆结瘤,种植作物比接种根瘤菌对土壤细菌群落的影响更大。
In order to study the influence of S.fredii WGF03 and its exoD gene mutant on the nodulation and soil microbial community in soybean and to further understand the function of exoD gene, the nodule of soybean per plant was collected at the flowering stage and counted. Analysis of soil microbial community by DGGE electrophoresis combined with sequencing. The results showed that after inoculation of S.fredii WGF03, the number of nodules increased by 191.67% compared with the non-inoculated group. Soybean inoculation with exo D mutants had the least number of nodules, which was 16.67% less than that in non-inoculation groups. Compared with the blank, the species and quantity of soil bacteria increased significantly after inoculation of soybean. After inoculation of different rhizobia, bacterial species and bacterial diversity also changed. Sequencing results showed that the bacteria in the soil were mainly Proteobacteria, accounting for 45.5% Indigenous rhizobia Bradyrhizobium. In summary, S.fredii WGF03 promoted soybean nodulation, and crop production had a greater impact on soil bacterial communities than Rhizobium inoculation.