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目的:探讨凋亡抑制因子Bcl-2与Cox-2在宫颈癌、宫颈上皮内瘤变(CIN)、宫颈炎组织中的表达情况,评估两者作为免疫组化指标在宫颈病变诊断中的可能性。方法:收集140例具有阴道镜检查指征患者的宫颈组织,采用免疫组化SP方法检测Bcl-2和Cox-2的表达。结果:140例患者中有60例宫颈炎、56例宫颈上皮内瘤变、24例宫颈癌,Bcl-2表达阳性率分别为13.3%、53.6%和91.7%;Cox-2表达阳性率分别为30.0%、64.3%及100.0%。Bcl-2及Cox-2在宫颈炎、CINⅠ、CINⅡ-III和宫颈癌中的表达率总体差异均有统计学意义(P<0.05),组间两两比较的差异亦具有统计学意义(P<0.008)。Bcl-2和Cox-2的表达呈正相关(rs=0.779,P<0.0001)。结论:凋亡抑制因子Bcl-2与Cox-2应用于宫颈癌及癌前病变的诊断和鉴别诊断中,对提高诊断的准确率有一定的临床意义。
OBJECTIVE: To investigate the expression of Bcl-2 and Cox-2 in cervical cancer, cervical intraepithelial neoplasia (CIN) and cervicitis and to evaluate their potential role in the diagnosis of cervical lesions as immunohistochemical markers Sex. Methods: The cervical tissue of 140 patients with indications of colposcopy was collected. The expression of Bcl-2 and Cox-2 was detected by immunohistochemical SP method. Results: 60 cases of cervicitis, 56 cases of cervical intraepithelial neoplasia and 24 cases of cervical cancer were found in 140 cases. The positive rates of Bcl-2 were 13.3%, 53.6% and 91.7% respectively. The positive rates of Cox-2 were 30.0%, 64.3% and 100.0%. There were significant differences in the expression rates of Bcl-2 and Cox-2 between cervicitis, CINⅠ, CINⅡ-III and cervical cancer (P <0.05), and the difference between groups was also statistically significant (P <0.008). The expressions of Bcl-2 and Cox-2 were positively correlated (rs = 0.779, P <0.0001). Conclusion: The apoptosis inhibitor Bcl-2 and Cox-2 are used in the diagnosis and differential diagnosis of cervical cancer and precancerous lesions, which have certain clinical significance in improving the diagnostic accuracy.