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目的本研究探讨体外培养大鼠胚胎皮肤成纤维细胞和人胚胎皮肤成纤维细胞内Ⅰ、Ⅲ型胶原的表达及意义。方法分别从大鼠胚胎(孕14d)和人胚胎(孕20周),获得原代的胚胎皮肤成纤维细胞。将第三代细胞荧光量子点标记技术检测其内Ⅰ、Ⅲ型胶原的表达。结果培养的第三代大鼠胚胎皮肤成纤维细胞和人胚胎皮肤成纤维细胞具有相同的显微结构,量子点标记检测其I、Ⅲ型胶原表达的平均光密度比较,差异无统计学意义(P﹥0.05)。结论体外培养第三代大鼠胚胎皮肤成纤维细胞和人胚胎皮肤成纤维细胞具有相同的形态结构及合成I、Ⅲ型胶原的能力相似,因此可能在组织无瘢痕愈合的修复过程中发挥相同的功能。
Objective This study was to investigate the expression and significance of type Ⅰ and type Ⅲ collagen in cultured rat embryonic dermal fibroblasts and human embryonic dermal fibroblasts. Methods Primary embryonic dermal fibroblasts were obtained from rat embryos (14th day of pregnancy) and human embryos (20th week of pregnancy) respectively. The third generation of cells fluorescence quantum dot labeling technique to detect the type Ⅰ, Ⅲ collagen expression. Results The cultured third generation rat embryonic dermal fibroblasts and human embryonic dermal fibroblasts had the same microstructure. There was no significant difference in the mean optical density of the type I and type III collagen detected by quantum dot labeling P> 0.05). CONCLUSION: The third generation of rat embryonic dermal fibroblasts and human embryonic dermal fibroblasts cultured in vitro have the same morphological structure and similar ability to synthesize type I and type III collagen, and therefore may play the same role in the repair process of tissueless scar healing Features.