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目的建立反相高效液相色谱法同时测定叶下珠中没食子酸、柯里拉京和短叶苏木酚的含量。方法采用Kromasil C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-体积分数0.1%磷酸水溶液梯度洗脱,流速1.0 mL.min-1,检测波长为280 nm,柱温30℃。结果没食子酸、柯里拉京和短叶苏木酚的质量浓度分别在5.300～106.7 mg.L-1(r=0.999 8)、2.00～40.6 mg.L-1(r=0.999 7)、0.270～5.42 mg.L-1(r=0.999 7)内与峰面积呈良好的线性关系。没食子酸、柯里拉京和短叶苏木酚的平均加样回收率(n=9)分别为97.9%、101.7%、100.2%,RSD分别为1.8%、1.8%、2.7%。结论该分析方法准确可靠,重复性好,为更好地控制叶下珠药材的质量提供方法。
Objective To establish an RP-HPLC method for simultaneous determination of gallic acid, corilagin and short-leaf hematoxylin in Phyllanthus urinaria. Methods A Kromasil C18 column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase consisted of a gradient of 0.1% phosphoric acid in acetonitrile with a flow rate of 1.0 mL · min-1. The detection wavelength was 280 nm and the column temperature was 30 ℃. Results The concentrations of gallic acid, corilagin and short-leaf hematoxylin were 5.300-106.7 mg.L-1 (r = 0.999 8), 2.00-40.6 mg.L-1 (r = 0.999 7) and 0.270-5.42 mg .L-1 (r = 0.999 7) and the peak area showed a good linear relationship. The mean recoveries of gallic acid, corilagin and short-leaf hematoxylin were 97.9%, 101.7% and 100.2%, respectively, with RSDs of 1.8%, 1.8% and 2.7%, respectively. Conclusion The method is accurate, reliable and reproducible. It provides a method for better controlling the quality of Phyllanthus urinaria.