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部分病毒性肝炎病人常出现明显贫血症状,有人曾认为可能与病毒侵犯骨髓有关。肝脏虽是乙肝病毒侵犯的主要靶器官,但各种类型肝组织的体外培养均未成功。乙肝病毒对骨髓感染能力如何,国内尚未报道。我们对新乡市传染病院的3名肝炎患者进行了HBV 感染骨髓可能性的调查。我们用 RPHA 法测定血清和骨髓浆中的HBsAg,并用免疫荧光抗体染色法(DIC)测定了骨髓细胞浆内的 HBsAg。骨髓和血细胞涂片均用冷丙酮固定,按常规二步抗体
Some patients with viral hepatitis often have obvious symptoms of anemia, some people think that may be related to the virus and bone marrow violations. Although the liver is the main target organ of hepatitis B virus invasion, all kinds of liver tissue have not been successfully cultured in vitro. Hepatitis B virus infection on the ability of the marrow, the country has not yet been reported. We investigated the possibility of HBV infection of bone marrow in 3 hepatitis patients in Xinxiang Infectious Hospital. We measured HBsAg in serum and bone marrow pulp by RPHA method and HBsAg in bone marrow cytoplasm by immunofluorescence staining (DIC). Bone marrow and blood smears were fixed with cold acetone, according to conventional two-step antibody