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为明确乙肝病毒对骨髓中三种细胞的作用 ,用乙型肝炎病毒 (HBV)体外感染正常人骨髓中的淋巴细胞、中性粒细胞和单核细胞。培养 6小时至 3周后行动态观察 ,乙型肝炎核心抗原 (HBc Ag)阳性率表明 ,三种细胞均受到不同程度的感染 ,以中性粒细胞和单核细胞最敏感 ,其次为淋巴细胞 ,三者间有统计学差异 (P<0 .0 5或0 .0 1)。病毒感染程度随细胞增殖和时间延长而增强 ,无吞噬能力的淋巴细胞亦是如此。透射电镜观察感染后的中性粒细胞 ,6小时即出现线粒体和内质网的空泡变和髓样变性 ,2 4小时细胞膜破裂 ,72小时细胞表面重度破坏。表明病毒感染细胞有两种途径 ,一为病毒主动侵犯细胞 ,二为细胞吞噬病毒 ,在不同时期培养的上清液中均长时期检测到乙肝病毒表面抗原 (HBs Ag)、乙型肝炎病毒 e抗原 (HBe Ag)和乙型肝炎病毒 DNA (HBV- DNA)。
To clarify the effect of hepatitis B virus on three types of cells in the bone marrow, hepatitis B virus (HBV) was used to infect lymphocytes, neutrophils and monocytes in normal human bone marrow in vitro. After 6 hours to 3 weeks of incubation, the positive rate of HBcAg showed that all three kinds of cells were infected to varying degrees, most sensitive to neutrophils and monocytes, followed by lymphocytes, There was a statistically significant difference (P <0.05 or 0.01). The extent of virus infection increases with cell proliferation and prolongation of time, as does non-phagocytic lymphocytes. The infected neutrophils were observed by transmission electron microscopy. The vacuoles and myeloid degeneration of mitochondria and endoplasmic reticulum were observed in 6 hours, the cell membrane ruptured in 24 hours and the surface of cells were severely damaged in 72 hours. The results showed that there are two ways for the virus to infect the cells. One is the virus aggressively invades the cells and the other is the phagocytosis of the virus. The supernatants cultured in different periods have detected HBsAg, Antigen (HBeAg) and Hepatitis B virus DNA (HBV-DNA).