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目的探索Pin X1基因在胃癌中的表达情况,并观察Pin X1表达水平改变对胃癌细胞恶性生物学行为的影响。方法通过Pin X1质粒转染及RNAi干扰技术,分别上调和下调胃癌细胞中的Pin X1基因表达水平,然后运用Western Blot技术在蛋白水平检测正常胃癌细胞、Pin X1基因上调组和Pin X1基因敲低组胃癌细胞目标蛋白Pin X1的表达情况,并通过CCK8法检测不同处理组胃癌细胞增殖曲线。选取36对胃癌组织和癌旁正常胃黏膜的组织,检测Pin X1的表达情况并计算其与肿瘤分期之间的相关性。采用SPSS软件进行统计分析并绘制统计图。结果胃癌细胞系SGC7901及MKN28中Pin X1的表达水平低于正常胃黏膜细胞株GES-1中Pin X1的表达水平,Pin X1基因在MKN28转染组胃癌细胞较正常MKN28细胞中表达上调,MKN28胃癌细胞生长速度相应的减缓(P<0.05),而干扰组胃癌细胞Pin X1基因表达下调,SGC7901胃癌细胞生长速度相应的增快(P<0.05)。Pin X1在胃癌组织中的表达率低于癌旁组织的表达率(P<0.05),且Pin X1表达水平与胃癌T分期呈现出一定的相关性。结论 Pin X1在胃癌组织和胃癌细胞中低表达并负性调控胃癌细胞恶性生物学行为。
Objective To explore the expression of Pin X1 gene in gastric cancer and to observe the influence of Pin X1 expression on the malignant behavior of gastric cancer cells. Methods The Pin X1 plasmid was transfected into human gastric cancer cells by RNA interference technique. Pin X1 gene was upregulated and Pin X1 knockdown was detected by Western Blot. The target protein Pin X1 of gastric cancer cells was detected by CCK8 method. The proliferation curves of gastric cancer cells in different treatment groups were detected. Thirty-six pairs of gastric cancer tissues and adjacent normal gastric mucosa tissues were selected to detect Pin X1 expression and calculate their correlation with tumor staging. Using SPSS software for statistical analysis and drawing charts. Results The expression level of Pin X1 in gastric cancer cell lines SGC7901 and MKN28 was lower than that in normal gastric mucosal cell line GES-1. Pin X1 gene was up-regulated in MKN28 transfected gastric cancer cells compared with normal MKN28 cells. MKN28 gastric cancer (P <0.05). However, Pin X1 gene expression was down-regulated in SGC7901 gastric cancer cells, and the growth rate of SGC7901 cells was correspondingly increased (P <0.05). The expression of Pin X1 in gastric cancer tissues was lower than that in paracancerous tissues (P <0.05), and Pin X1 expression was correlated with T stage of gastric cancer. Conclusion Pin X1 is low expressed in gastric cancer and gastric cancer cells and negatively regulates the malignant behavior of gastric cancer cells.