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【目的】探讨siRNA干扰肺特异性X蛋白（LUNX）对非小细胞肺癌（NSCLC）细胞系A549增殖和侵袭的影响。【方法】采用脂质体LipofectamineTM 2000介导LUNX siRNA转染人NSCLC细胞系A549，转染48 h后分别用 qRT‐PCR和 Western blot方法评价干扰效果；MTT法检测LUNX siRNA转染对细胞增殖的影响；Transwell法检测LUNX siRNA转染对细胞侵袭能力的影响；并用 qRT‐PCR 和 Western blot方法检测p‐AKT的表达。【结果】转染后NSCLC细胞系A549内LUNX的表达显著降低；LUNX表达降低使NSCLC细胞系A549的增殖减慢及侵袭能力降低，且LUNX表达降低后p‐AKT的mRNA和蛋白表达均显著降低。【结论】siRNA干扰后LUNX表达、NSCLC细胞系的增殖和侵袭能力降低，可能与其表达降低后p‐AKT表达减少相关。“,”Objective]To explore the effects of siRNA targeting Lunx on the proliferation and invasiveness of non‐small cell lung cancer cell line A549 (NSCLC) .[Methods]siRNA Lunx was transfected into non‐small cell lung cancer cell line with Lipofectamine TM2000 .At 48h post‐transfection ,quantitative real‐time poly‐merase chain reaction (qRT‐PCR) and Western blot were used to assay the inhibitory effect of Lunx SiRNA on the expression of Lunx .The cell proliferation of NSCLC was determined by thiazolyl blue tetrazolium blue (MTT) .The invasiveness of NSCLC cell line was determined by Transwell .And the altered expression of p‐AKT was detected by qRT‐PCR and Western blot .[Results]After 48‐hour transfection with Lunx SiRNA , the expressions of Lunx mRNA and protein in NSCLC cell line became inhibited significantly .After transfec‐tion ,the proliferation capacities of NSCLC cell line decreased significantly .Compared with control siRNA ,the invasiveness of NSCLC cell line decreased significantly .And the expression of p‐AKT decreased significantly .[Conclusion]By siRNA interference ,the expression of Lunx gene becomes effectively inhibited .And silencing Lunx gene may decrease cell invasiveness through a down‐regulated expression of p‐AKT .