以肝素抗疑,54万分子量葡聚糖助沉红细胞,获得原白细胞数77.9±O.2％。经尼龙丝柱法分离的白细胞,用五种方法检查细胞活力(细胞活动力、苔盼兰排出试验、伊红漆色、吖啶橙体外活体染色、吞噬力)6小时白细胞阿米巴运动仍很活跃。比较了尼龙丝柱法与低渗法分离之淋巴细胞及中性粒细胞的数量和质量,30毫升全血,经尼龙丝柱分离法分离之中性柱细胞,其数量(10~7～10~8)和质量均可满足本研究标记白细胞之用。 Heparin anti-suspect, 540,000 molecular weight dextran aid deposition of erythrocytes, the number of white blood cells obtained 77.9 ± O. 2%. Leukocytes isolated by nylon filament column method were examined for cell viability by five methods (cell viability, trypan blue discharge test, erythroid lacquer, acridine orange in vitro staining, phagocytosis) for 6 hours. Very active. The number and quality of lymphocytes and neutrophils isolated from nylon and hypotonic methods were compared. Neutrophils were isolated from 30 ml of whole blood and separated by nylon filament columns. The number of cells (10 ~ 7 ~ 10 ~ 8) and the quality of the study can be used to mark the white blood cells.