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8个医疗单位外环境及医疗器械采集混合样本187份,乙型肝炎表面抗原(HBsAg)阳性率4.48％,且阳性率随着被血液污染程度的增加而上升。“常规消毒”后医疗器械仍有2.78％阳性。1～2％新洁尔灭及1～2％过氧乙酸对高滴度(1:25 600)HBsAg无作用,但对低滴度(1:3200)有一定作用。现场消毒液浓度符合率仅60％。采集消毒液经超离心后9只样品中有8只HBsAg阳性,电镜下见到乙型肝炎病毒(HBV)三种颗粒:球形、管形及Dane颗粒。提示某些医疗单位具有乙肝医源性传播的可能性。
A total of 187 mixed samples were collected from 8 medical units outside the environment and medical devices. The positive rate of HBsAg was 4.48%, and the positive rate increased with the increase of blood pollution. After “routine disinfection” medical devices still 2.78% positive. 1 to 2% benzalkonium chloride and 1 to 2% peracetic acid have no effect on high titer (1: 25600) HBsAg, but have a certain effect on low titer (1: 3200). On-site disinfectant concentration in line with the rate of only 60%. Eight of the nine samples collected after ultracentrifugation were positive for HBsAg. Three particles of hepatitis B virus (HBV) were observed under electron microscopy: spherical, tubular and Dane particles. Suggesting some medical units have the possibility of iatrogenic hepatitis B transmission.