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目的制备和表征肿瘤热疗用磁流体。方法在聚乙二醇6000(PEG-6000)的存在下用化学共沉淀法制备肿瘤热疗用四氧化三铁(Fe3O4)磁流体,用邻二氮菲显色法测定磁流体中铁的含量,通过电子显微镜、X衍射、红外和振动样品磁强计对制备的磁流体进行表征。测定了磁流体在交变磁场作用下的热效应,并将该磁流体用于VX2兔肿瘤的热疗。结果红外图谱和X衍射图谱证明所制备的磁流体样品为Fe3O4;电镜照片显示磁性粒子近乎圆形且分散良好;经X衍射数据计算得磁性粒子的粒径为13.3±3.8nm;样品的饱和磁化强度和剩余磁化强度分别为23.39A/m(1.556emu/g)和0.56A/m(0.02604emu/g),矫顽力为12Oe;磁流体的特征吸收率为69±10W/g[Fe]。将该磁流体直接注射于VX2兔肝肿瘤部位后,置于交变磁场中进行热疗,测得肿瘤部位温度可达到41~46°C。结论在PEG-6000存在下所制备的Fe3O4磁流体有望用于肿瘤热疗。
Objective To prepare and characterize the magnetic fluids for tumor hyperthermia. Methods Ferroferric oxide (Fe3O4) was prepared by chemical coprecipitation in the presence of polyethylene glycol 6000 (PEG-6000). The content of Fe in the ferrofluid was determined by phenanthroline colorimetry. The prepared magnetic fluids were characterized by electron microscope, X-ray diffraction, infrared and vibrational sample magnetometers. The thermal effect of the magnetic fluid under the action of an alternating magnetic field was measured, and the magnetic fluid was used for hyperthermia of VX2 rabbit tumors. Results The X-ray diffraction and X-ray diffraction confirmed that the magnetic fluid sample was Fe3O4. The electron microscopy showed that the magnetic particles were nearly circular and well dispersed. The diameter of the magnetic particles was 13.3 ± 3.8nm as calculated by X-ray diffraction data. The saturation magnetization The strength and residual magnetization were 23.39 A / m (1.556 emu / g) and 0.56 A / m (0.02604 emu / g) respectively, and the coercive force was 12 Oe. The characteristic absorption of the magnetic fluid was 69 ± 10 W / g [Fe] . The magnetic fluid injected directly into the VX2 rabbit liver tumor site, placed in an alternating magnetic field for hyperthermia, the measured tumor site temperature can reach 41 ~ 46 ° C. Conclusion Fe3O4 magnetic fluid prepared in the presence of PEG-6000 is expected to be used in tumor hyperthermia.