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目的:探讨小细胞肺癌(SCLC)组织和小细胞肺癌细胞(H446)中肌糖蛋白-C(TN-C)的表达及STAT3对TN-C表达的影响。方法:应用免疫组化法检测58例小细胞肺癌和17例癌旁正常组织中TN-C的表达水平,应用RT-PCR和Western blotting法检测STAT-siRNA和STAT3过表达的H446细胞中TN-C的表达水平。结果:(1)小细胞肺癌组织中TN-C的表达水平显著高于癌旁正常组织(P<0.05);(2)在H446细胞中,TN-C和STAT3均呈现高表达;(3)STAT3-siRNA处理的H446细胞中STAT3和TN-C的表达均显著降低(P<0.05),而STAT3过表达的H446细胞中STAT3和TN-C的表达均显著上调(P<0.05)。结论:TN-C在小细胞肺癌中的表达上调,可能受到STAT3的调控。
Objective: To investigate the expression of muscle glyco-protein C (TN-C) in small cell lung cancer (SCLC) and small cell lung cancer cells (H446) and the effect of STAT3 on TN-C expression. Methods: The expression of TN-C in 58 small cell lung cancer tissues and 17 adjacent normal tissues was detected by immunohistochemistry. The expression of TN-C in STAT-siRNA and STAT3-overexpressing H446 cells was detected by RT-PCR and Western blotting. C expression level. Results: (1) The expression of TN-C in small cell lung cancer tissues was significantly higher than that in adjacent normal tissues (P <0.05); (2) TN-C and STAT3 were highly expressed in H446 cells; The STAT3 and TN-C expression in H446 cells treated with STAT3-siRNA decreased significantly (P <0.05), while STAT3 and TN-C expression in STAT3-siRNA-treated H446 cells increased significantly (P <0.05). Conclusion: The up-regulation of TN-C in small cell lung cancer may be regulated by STAT3.