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[目的]茚虫威是一种新型二嗪类杀虫剂,作用机制独特,探讨了斜纹夜蛾对茚虫威的抗性风险和抗性机理。[方法]采用饲料浸毒法对斜纹夜蛾敏感种群3龄幼虫经过13代11次抗性选育,测定斜纹夜蛾对茚虫威的抗性倍数及抗性机理。[结果]斜纹夜蛾对茚虫威的抗性倍数达到69.6倍。测定选育抗性种群3龄幼虫羧酸酯酶、谷胱甘肽S-转移酶和多功能氧化酶活性结果表明:选育种群羧酸酯酶活性和多功能氧化酶O-脱甲基活性分别是选育前的3.7倍和2.5倍,差异显著;谷胱甘肽S-转移酶为选育前的1.1倍,差异不显著。[结论]斜纹夜蛾对茚虫威的抗药性与羧酸酯酶和多功能氧化酶活性有关,与谷胱甘肽S-转移酶无关。
[Objective] Indoxacarb is a novel diazine insecticide with unique mechanism of action and its mechanism of resistance to indoxacarb and the mechanism of resistance to indoxacarb were discussed. [Method] The 3rd instar larvae of the susceptible population of Spodoptera litura were eleven times of eleven times breeding with feed digestion method to determine the resistance multiples and the mechanism of resistance to indoxacarb in S. litura. [Result] The resistance multiples of Spodoptera litura to indoxacarb reached 69.6 times. The activities of carboxylesterase, glutathione S-transferase and polyfunctional oxidase of 3rd instar larvae of selected resistant populations were determined. The results showed that carboxylesterase activity and multi-functional oxidase O-demethylation activity Respectively, 3.7 and 2.5 times before breeding, the difference was significant; glutathione S-transferase 1.1 times before breeding, the difference was not significant. [Conclusion] The resistance of Spodoptera litura to indoxacarb was related to the activity of carboxylesterase and polyfunctional oxidase, but not glutathione S-transferase.