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研究了磷钼杂多酸修饰电极对去甲肾上腺素(NE)、肾上腺素(E)和多巴胺(DA)等儿茶酚胺类神经递质的催化氧化作用,探讨了催化机理,并采用高压液相色谱电化学方法对其进行了分离检测,NE、DA和E的线性范围(mol)依次为8.0×10~(-11)～2.0×10~(-8)、8.0×10~(-11)～2.0×10~(-8)、4.0×10~(-11)～2.0×10~(-8);检测限(mol))依次为4.0×10~(-11)、4.0×10~(-11)、2.0×10~(-11).7次平行测定的相对标准偏差(％)依次为1.6、2.0和4.5.将此法用于鼠脑组织中神经递质的测定,获得满意的结果.
The catalytic oxidation of catecholamine neurotransmitters such as norepinephrine (NE), epinephrine (E) and dopamine (DA) by the phosphomolybdic heteropoly acid modified electrode was studied. The catalytic mechanism was explored. High pressure liquid chromatography The linear range (mol) of NE, DA and E were 8.0 × 10 ~ (-11) ~ 2.0 × 10 ~ (-8), 8.0 × 10 ~ (-11) ~ 2.0 × 10 ~ (-8), 4.0 × 10 ~ (-11) ~ 2.0 × 10 ~ (-8), detection limit (mol)) were 4.0 × 10 ~ (-11), 4.0 × 10 ~ 11) and 2.0 × 10 ~ (-11). The relative standard deviations (%) of the seven parallel determinations were 1.6, 2.0 and 4.5, respectively. This method was applied to the determination of neurotransmitters in rat brain tissue with satisfactory results .