论文部分内容阅读
目的 探讨肾综合征出血热 (HFRS)早期诊断方法。方法 用MacELISA、IFAT二种方法同时检测HFRS病人血清40份 ;非出血热血清20份 ;正常人血清20份进行对比研究。而且 ,阳性血清用2 -巯基乙醇破坏特异性IgM试验 ,阴性血清加类风湿因子阳性血清干扰试验。 结果 用MacELISA法检测HFRS于病程第2日即可出现特异性IgM阳性 ,3～4病日阳性率达89 % ;5～7病日阳性率达100% ,而IFAT法时病日抗体阳性率为55%与70% (P<0.05)。非出血热病人与正常人特异性抗体全部阴性。阳性血清用2 -巯基乙醇破坏IgM后MacELISA检测为阴性 ,阴性血清加类风湿阳性血清后用本法检测无假阳性。结论 两法相比 ,MacELISA法诊断HFRS特异性强 ,灵敏度高 ,早期诊断意义大
Objective To investigate the early diagnosis of hemorrhagic fever with renal syndrome (HFRS). Methods MacELISA and IFAT were used to detect 40 serum samples of HFRS patients at the same time; 20 non-hemorrhagic fever serum samples and 20 normal serum samples were compared. Moreover, the positive serum with 2 - mercaptoethanol destroys the specific IgM test, negative serum plus rheumatoid factor positive serum interference test. Results The positive rate of HFRS detected by MacELISA method on the 2nd day of the course of disease was up to 89%, and the positive rate was 100% on the 5th to 7th day. The IFAT positive rate 55% and 70% (P <0.05). Non-hemorrhagic fever patients and normal all the specific antibodies. Positive serum with 2 - mercaptoethanol after IgM MacELISA test was negative, negative serum plus rheumatoid positive serum with this method to detect no false positive. Conclusion Compared with the two methods, MacELISA diagnosis of HFRS specific, high sensitivity, early diagnosis of great significance