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将用合成的匙孔形虫戚血蓝蛋白－甲醛－河豚毒素（ＫＬＨ－ＨＣＨＯ－ＴＴＸ）连接物免疫的ＢＡＬＢ／ｃ小鼠的脾细胞与小鼠骨髓瘤细胞系Ｓｐ２／０融合，经３～４次亚克隆，建立了３个稳定分泌抗河豚毒素的杂交瘤细胞株，分别命名为１Ｇ３、４Ｇ３、６Ｄ９。其中１Ｇ３和４Ｇ３分属ＩｇＧ２ｂ亚类，６Ｄ９属ＩｇＧ１亚类。腹水的抗体稀释度为１∶１０５～１：５×１０６。用竞争抑制性酶联免疫吸附试验（ＥＬＩＳＡ）测定三种抗体的最低反应浓度为１×１０－３ｍｇ／Ｌ。
Spleen cells of BALB / c mice immunized with the synthetic keyhole limpet hemocyanin-formaldehyde-tetrodotoxin (KLH-HCHO-TTX) conjugate were fused with the mouse myeloma cell line Sp2 / 0 via 3 ~ 4 subclones, established three stable secreted anti-tetrodotoxin hybridoma cell lines, respectively, named 1G3, 4G3, 6D9. Among them, 1G3 and 4G3 belong to IgG2b subclass and 6D9 belong to IgG1 subclass. Ascites antibody dilution 1:105 ~ 1: 5 × 106. The minimum reaction concentration of the three antibodies was 1 × 10-3 mg / L as determined by competitive inhibition enzyme-linked immunosorbent assay (ELISA).