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目的:用HPLC法测定同时间同年生同生长土壤铜陵和亳州的牡丹皮中丹皮酚和芍药苷的含量,并比较其质量差异。方法:色谱柱:Waters VP-ODS-C18(150 mm×4.6 mm,5μm);丹皮酚含量测定流动相:甲醇:水(60∶40),检测波长:274 nm,柱温:30℃,流速1.0 mL·min-1;芍药苷含量测定流动相:乙腈:0.1%H3PO4(20∶80);检测波长:230 nm;柱温:30℃;流速:1.0 mL·min-1。结果:丹皮酚在1.073-5.365μg,芍药苷在0.593-2.965μg范围内,均呈良好线性关系,相关系数分别为0.9999、0.9991,平均回收率丹皮酚为99.96%(RSD%=0.72%),芍药苷为99.03%(RSD%=0.49%)。结论:在选定的牡丹皮中丹皮酚的含量,铜陵产区远高于亳州产区;而芍药苷的含量,铜陵产区略低于亳州产区。
OBJECTIVE: To determine the contents of paeonol and paeoniflorin in the moutan barks of Tongling and Bozhou, the same year of the same year by HPLC, and to compare their differences in quality. Method: Column: Waters VP-ODS-C18 (150 mm × 4.6 mm, 5 μm); Paeonol content was measured by mobile phase methanol: water 60:40, detection wavelength 274 nm, column temperature 30 ℃, Flow rate 1.0 mL · min-1. Determination of paeoniflorin Mobile phase: Acetonitrile: 0.1% H3PO4 (20:80); Detection wavelength: 230 nm; Results: Paeonol had a good linearity in the range of 1.073-5.365μg and paeoniflorin in the range of 0.593-2.965μg with the correlation coefficients of 0.9999 and 0.9991, respectively. The average recovery was 99.96% (RSD% = 0.72% ), Paeoniflorin was 99.03% (RSD% = 0.49%). CONCLUSION: The content of paeonol in selected moutan bark is far higher in Boiling area than in Bozhou producing area. The content of paeoniflorin in Tongling producing area is slightly lower than that in Bozhou producing area.