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目的:建立液相色谱-串联质谱法测定人血浆中奥美拉唑的血药浓度并进行药动学研究。方法:以兰索拉唑为内标,血浆样品经乙腈沉淀后,经LC-MS/MS分离分析。采用Diamonsil C18柱(2.1 mm×150 mm,5μm),甲醇-水(含0.01%甲酸)(67∶33)为流动相;流速为0.3 ml·min-1,采用电喷雾离子源(ESI),以多反应监测方式(MRM)进行负离子监测,奥美拉唑和兰索拉唑的定量分析离子对分别为m/z 344.2/193.8,367.9/163.8。结果:奥美拉唑在3.38~2 110.00ng·ml-1(r=0.997 1)范围内线性关系良好,最低定量限为3.38 ng·ml-1,方法回收率在92.48%~99.39%,日内(n=5)RE均小于3.96%,日间(n=15)RE均小于6.41%。结论:该方法快速简便,灵敏准确,可用于奥美拉唑血药浓度监测和药动学研究。
Objective: To establish a liquid chromatography - tandem mass spectrometry determination of plasma concentrations of omeprazole in human plasma and pharmacokinetic studies. Methods: Lansoprazole was used as an internal standard. The plasma samples were separated by acetonitrile and analyzed by LC-MS / MS. The mobile phase consisted of Diamonsil C18 column (2.1 mm × 150 mm, 5 μm), methanol-water (containing 0.01% formic acid) (67:33) and flow rate of 0.3 ml · min -1. Electrospray ionization (ESI) Negative ion monitoring was performed in multiple reaction monitoring (MRM) mode. Quantitative analysis of omeprazole and lansoprazole ion pairs was m / z 344.2 / 193.8, 367.9 / 163.8, respectively. Results: The linear range of omeprazole in the range of 3.38-2 110.00 ng · ml-1 (r = 0.997 1) was good, with the lowest limit of quantification of 3.38 ng · ml-1 and the recovery of the method was 92.48% -99.39% (n = 5) REs were all less than 3.96%. Day RE (n = 15) REs were all less than 6.41%. Conclusion: The method is rapid, simple, sensitive and accurate and can be used for monitoring omeprazole concentration and pharmacokinetics.