论文部分内容阅读
建立了高效液相色谱-串联三重四极杆质谱法(HPLC-MS/MS)同时测定发泡塑料餐具中5种荧光增白剂(FWA135、FWA184、FWA367、FWA368、FWA393)的方法。样品以三氯甲烷溶解提取,加入甲醇使基质沉淀,提取液氮吹浓缩,使用PHENOMENEX KINETEX C18色谱柱,0.1%甲酸甲醇-0.1%甲酸水为流动相进行梯度洗脱分离,采用串联质谱ESI正模式电离,多反应监测(MRM)模式检测,以保留时间及离子对比较定性,外标法定量。结果表明,5种荧光增白剂在0.5~50μg/L范围内线性关系良好,相关系数大于0.995,仪器检出限(S/N=3)为0.1~0.5μg/L,方法定量限(S/N=10)为0.4~2.0μg/kg,添加水平为2、8、200μg/kg时,样品的回收率在86.4~98.4%之间,相对标准偏差(RSD,n=6)小于3.9~8.1%。该法前处理简单、回收率高、精密度好,适用于发泡塑料餐具中5种荧光增白剂的测定。
A method for the simultaneous determination of five fluorescent whitening agents (FWA135, FWA184, FWA367, FWA368, FWA393) in foamed plastic tableware by high performance liquid chromatography-tandem triple-quadrupole mass spectrometry (HPLC-MS / MS) was established. The sample was extracted with chloroform, precipitated by adding methanol, the extract was concentrated by nitrogen and concentrated. The residue was separated by gradient elution on a PHENOMENEX KINETEX C18 column with 0.1% formic acid-0.1% formic acid as mobile phase. Mode ionization, multiple reaction monitoring (MRM) mode detection, with retention time and ion pair more qualitative, external standard quantitative. The results showed that the linearity of the five fluorescent whitening agents in the range of 0.5-50 μg / L was good, the correlation coefficient was greater than 0.995, the detection limit (S / N = 3) was 0.1-0.5 μg / L and the limit of quantification (RSD, n = 6) was less than 3.9 ~ 4.0μg / kg, the recovery was between 86.4% and 98.4% at the concentration of 2,8,200μg / 8.1%. The method pretreatment is simple, high recovery, good precision, suitable for the determination of five kinds of optical brighteners in foamed plastic tableware.