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目的:探讨丹酚酸A对成纤维细胞增殖及胶原合成的影响。方法:常规培养NIH/3T3成纤维细胞,10~(-4)～10~(-7)mol/L丹酚酸A温育亚单层细胞22h。[~3H]-TdR掺入法测定细胞增殖,[~3H]-proline掺入法测定细胞活力,[~3H]-proline掺入、胶原酶消化法测定细胞内外胶原生成率。结果:10~(-4)～10~(-6)mol/L丹酚酸A抑制细胞增殖,除10~(-4)mol/L丹酚酸A组细胞[~3H]-proline掺入量下降,有一定细胞毒性外,其余各组均有促进细胞活力的趋势,以10~(-6)mol/L组最为明显。10~(-4)～10~(-7)mol/L丹酚酸A抑制细胞内胶原合成率,以10~(-6)mol/L作用稍强,但对细胞外胶原的分泌均无明显影响。结论:丹酚酸A能抑制成纤维细胞增殖及细胞内胶原合成,体外最佳作用浓度为10~(-6)mol/L。
Objective: To investigate the effect of salvianolic acid A on fibroblast proliferation and collagen synthesis. METHODS: NIH/3T3 fibroblasts were routinely cultured, and submonolayer cells were incubated with 10~(-4)~10~(-7) mol/L salvianolic acid A for 22 hours. [~3H]-TdR incorporation method was used to measure cell proliferation, [~3H]-proline incorporation method was used to measure cell viability, [~3H]-proline was incorporated, and collagenase digestion method was used to measure intracellular and extracellular collagen production. RESULTS: 10~(-4)~10~(6)mol/L salvianolic acid A inhibited cell proliferation, except for 10~(-4)mol/L salvianolic acid A group cells [~3H]-proline incorporation. With the decrease of the amount, there was a certain cytotoxicity, and the remaining groups all had the tendency to promote cell viability, with the most obvious in the 10~(-6) mol/L group. 10~(-4) mol/L-10(-7) mol/L salvianolic acid A inhibited the rate of intracellular collagen synthesis and was slightly stronger than 10~(-6) mol/L, but no secretion of extracellular collagen was observed. Obvious effect. Conclusion: Salvianolic acid A can inhibit fibroblast proliferation and intracellular collagen synthesis. The optimal concentration in vitro is 10~(-6) mol/L.