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应用RNA分离、纯化及反转录技术,对低温处理草菇菌丝体的cDNA进行分析,发现草菇菌丝在低温协迫的最初2h内存在基因表达的变化。
The RNA isolation, purification and reverse transcription techniques were used to analyze the mycotoxin cDNA of M. rufoschus. The results showed that there was gene expression change during the first 2 hours of hyphae co-cultivation.