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目的探讨血管紧张素原(AGT)M235T基因多态性与老年高血压及其合并糖尿病的关系。方法采用聚合酶链反应(PCR)及限制性片段长度多态性分析法对60名健康老年对照组、105例老年高血压组、38例老年高血压合并糖尿病组进行M235T基因多态性检测。结果 MT、TT基因型和T等位基因频率在老年高血压组分别为0.371、0.457和0.643,老年高血压合并糖尿病组分别为0.289、0.632和0.776,均明显高于健康老年对照组的0.167、0.133和0.217(均P<0.05);老年高血压组与老年高血压合并糖尿病组患者的MT、TT基因型差异均无统计学意义(均P>0.05),但T等位基因频率的差异有统计学意义(P<0.05)。结论 AGT基因的TT型是老年高血压的危险因素,但不是糖尿病的危险因素。
Objective To investigate the relationship between angiotensinogen (AGT) M235T gene polymorphism and senile hypertension and its associated diabetes mellitus. Methods Polymerase chain reaction (PCR) and restriction fragment length polymorphism (PCR-RFLP) were used to detect M235T polymorphism in 60 healthy elderly controls, 105 elderly hypertensive patients and 38 elderly hypertensive patients with diabetes mellitus. Results The frequencies of MT, TT genotypes and T allele were 0.371,0.457 and 0.643 respectively in the elderly hypertensive group and 0.289,0.632 and 0.776 in the elderly hypertensive diabetic group, respectively, which were significantly higher than those in the healthy control group (0.167, 0.133 and 0.217 respectively (all P <0.05). There was no significant difference in the genotypes of MT and TT between the elderly hypertensive group and the elderly patients with hypertension and diabetes mellitus (all P> 0.05) Statistical significance (P <0.05). Conclusion The TT type of AGT gene is a risk factor for hypertension in elderly patients, but it is not a risk factor for diabetes mellitus.