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目的探讨用梅毒螺旋体抗体酶联免疫吸附试验(TP-ELISA)联合梅毒甲苯胺红不加热血清试验(TRUST)和梅毒螺旋体明胶凝集试验(TPPA)的方法避免TP-ELISA法筛查梅毒抗体的假阳性结果。方法用TP-ELISA法作为临床标本的梅毒筛查实验,对阳性标本进一步作TRUST和TPPA检测。结果 TP-ELlSA法检出114例梅毒阳性标本,TPPA确证阳性111例,阳性符合率为97.36%(111/114);其中27例TRUST阳性,TPPA确证试验均为阳性,阳性符合率为100%;97例TRUST阴性,TPPA确证阳性94例,阴性3例,不符合率为2.64%。结论 TP-ELISA法筛查梅毒存在一定比例的假阳性结果,对TP-ELlSA法测出的阳性标本先做TRUST试验,再用TPPA确证,阳性结果报告梅毒抗体阳性,阴性结果报告梅毒抗体阴性,这样能有效避免单一TP-ELISA法的假阳性结果。
Objective To investigate whether TP-ELISA screening of syphilis antibodies by TP-ELISA combined with TRUST and TPPA assays Positive result. Methods TP-ELISA as a clinical specimen of syphilis screening experiments, the positive specimens further TRUST and TPPA detection. Results Totally 114 cases of syphilis were detected by TP-ELSA method. The positive rate of TPPA was 111.34% (111/114), of which 27 cases were TRUST-positive and confirmed by TPPA confirmatory test. The positive coincidence rate was 100% ; 97 cases of TRUST negative, TPPA confirmed positive in 94 cases, negative in 3 cases, the non-compliance rate of 2.64%. Conclusion TP-ELISA screening of syphilis has a certain proportion of false-positive results, TP-ELlSA method to do the first positive test TRUST test confirmed by TPPA confirmation, positive results report positive syphilis antibody, negative results report negative syphilis antibody, This effectively avoids the false-positive result of a single TP-ELISA.