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转化生长因子β(transforming growth factor-β,TGF-β)是一大类具有多种生物学功能的细胞因子,其对细胞增殖生长具有双向调节作用,既可作为促进细胞增殖的信号,又可抑制细胞生长。一般认为,其对上皮起源的细胞为一潜在的生长抑制因子,由于这一特性,目前普遍认为细胞对TGF-β负调控作用应答的丧失是肿瘤形成中的一个重要因素。本研究将通过免疫组织化学方法观察TGF-β及其受体在大肠癌中的分布、定位及表达水平,以探讨其在大肠癌形成中的作用。 1 材料和方法 1.1 材料随机收集解放军210医院1992/1996手术切除标本之石蜡切块,其中大肠癌56例,男34例,女22例,平均年龄72(41～81)岁,术前均未行化疗、放疗。组织学类型:腺癌42例,粘液癌7例,未分化癌7例;Dukes分期A期8例,B期16例,C期22例,D期10例,每例标本均收集其相应的癌旁组织
Transforming growth factor-β (TGF-β) is a large class of cytokines with various biological functions. It has a two-way regulatory effect on cell proliferation and growth, and can be used as a signal for promoting cell proliferation. Inhibit cell growth. It is generally believed that it is a potential growth inhibitory factor for cells of epithelial origin, and due to this characteristic, it is generally believed that the loss of cells’ response to the negative regulation of TGF-β is an important factor in tumor formation. In this study, the distribution, localization and expression of TGF-β and its receptors in colorectal cancer were observed by immunohistochemistry to explore its role in the development of colorectal cancer. 1 Materials and methods 1.1 Materials Randomly collected paraffin sections from surgical resection specimens of PLA 210 Hospital 1992/1996, including 56 cases of colorectal cancer, 34 males and 22 females, with an average age of 72 (41-81) years old. Chemotherapy and radiotherapy. Histological types: adenocarcinoma in 42 cases, mucinous carcinoma in 7 cases, undifferentiated carcinoma in 7 cases; Dukes stage A in 8 cases, B in 16 cases, C in 22 cases, D in 10 cases, each specimen was collected its corresponding Paracancer