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目的探讨脑膜癌病的脑脊液细胞学特征。方法对4例脑膜癌病患者行腰椎穿刺脑脊液细胞学检查,其中2例行免疫细胞化学检查。脑脊液细胞学检查采用粟氏玻片离心法,MGG染色,免疫细胞化学检查采用酶标聚合物法(LDP法)。结果 4例患者经脑脊液细胞学检查均发现肿瘤细胞,细胞成团聚集或散在分布,大小不一,形态各异,胞膜有突起,胞浆深染,核浆比例增大,核仁明显。免疫细胞化学检查示广谱角蛋白(CKpan)阳性1例。结论脑膜癌病的脑脊液细胞学主要特征为有异常形态的肿瘤细胞,这是脑膜癌病诊断的主要依据,结合免疫细胞化学检查有助于明确肿瘤细胞的性质。
Objective To investigate the cerebrospinal fluid cytological features of meningeal carcinomatosis. Methods Cytological examination of cerebrospinal fluid in lumbar puncture was performed in 4 cases of patients with meningeal carcinomatosis. Immunocytochemistry was performed in 2 of them. Cytological examination of cerebrospinal fluid was performed using glass slide centrifugation and MGG staining. Immunocytochemistry was performed by enzyme-linked polymer method (LDP method). Results Four patients were found by cerebrospinal fluid cytological examination of tumor cells, clusters or scattered distribution of cells, different sizes, morphological differences, cell membrane protrusions, deep cytoplasm, nuclear ratio increased nucleoli, nucleoli obvious. Immunocytochemistry showed that 1 case was positive for CKpan. Conclusion Cerebrospinal fluid cytology of meningeal carcinomatosis is characterized by abnormal morphology of tumor cells, which is the main basis for the diagnosis of meningeal carcinomatosis. Combined with immunocytochemistry, it is helpful to clarify the nature of tumor cells.