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消炎痛（０．００３－１．０μｍｏｌ／Ｌ）抑制由花生四烯酸（ＡＡ，３μｍｏｌ／Ｌ）诱导的家兔洗脱血小板细胞内游离钙浓度增加及血小板聚集反应，呈剂量依赖性。血栓烷Ａ２拮抗剂ＯＮＯ－３７０８（０．３．０μｍｏｌ／Ｌ）使ＡＡ的上述两个反应的量效曲线平行右移。另一种血栓烷Ａ２拮抗剂Ｓ１４５也非竞争性的抑制ＡＡ的上述反应。延长Ｓ－１４５的温培时间可增加Ｓ－１４５的抑制作用，另外Ｓ－１４５的抑制作用不能被冲洗掉，而ＯＮＯ－３７０８冲洗后其抑制作用消失。平行线测定的结果表明，以Ｓ－１４５处理后的洗脱血小板其ＡＡ量效曲线的斜率与Ｓ－１４５加ＯＮＯ－３７０８处理组的量效曲线并不平行，这一结果提示至少Ｓ－１４５和ＯＮＯ－３７０８很可能与同一受体的不同位点相结合，但分别与Ｓ－１４５和ＯＮＯ－３７０８结合的两种不同ＴＸＡ２／ＰＧＨ２受体存在的可能性依然存在。
Indomethacin (0.003-1.0 μmol / L) inhibited the increase of intracellular free calcium concentration and platelet aggregation induced by arachidonic acid (AA, 3 μmol / L) in a dose-dependent manner. The thromboxane A2 antagonist ONO-3708 (0.3.0 μmol / L) shifted the dose-response curves of the above two reactions of AA parallel to the right. Another thromboxane A2 antagonist, S145, also non-competitively inhibited the above reaction of AA. Prolonged S-145 incubation time increased the inhibitory effect of S-145. In addition, the inhibitory effect of S-145 could not be washed away, while the inhibitory effect disappeared after ONO-3708 washing. The results of parallel lines showed that the slope of AA dose-response curve of S-145-treated elution platelets was not parallel with the dose-response curve of S-145 plus ONO-3708 treatment group. This result suggests that at least S-145 And ONO-3708 are likely to bind to different sites on the same receptor, but the possibility of two different TXA2 / PGH2 receptors bound to S-145 and ONO-3708, respectively, still exists.