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目的探讨大豆甙元(daidzein)诱导H22肿瘤细胞凋亡的作用及机制。方法用四甲基偶氮噻唑蓝(MTT)法检测不同剂量大豆甙元(0.1～150.0μmol/L)对H22细胞增殖的影响;昆明雄性小鼠60只,随机分为6组,每组10只,腹腔注射给药,分别为模型组、对照组(不含血清的RPMI 1640培养液),低、中、高剂量大豆甙元组(大豆甙元分别为501、00、200 mg/kg),阳性对照组(环磷酰胺30 mg/kg);给药14 d后比较各组小鼠体重、脾脏指数、胸腺指数及瘤重;采用western blot检测bax、bcl-2的蛋白表达。结果 MTT检测结果显示大豆甙元对H22细胞有抑制作用,150μmol/L大豆甙元作用72 h,抑制率达30.3%,与低剂量组比较有明显抑制作用(P<0.05);大豆甙元能使Bax表达上调,Bcl-2表达下调,与对照组比较,10.0μmol/L大豆甙元组bax表达灰度值增加50.05%,凋亡抑制基因bcl-2表达减少52.62%;中、高剂量大豆甙元组小鼠胸腺指数和脾脏指数均明显升高(P<0.01),与模型组比较,大豆甙元各组瘤重指数下降明显(P<0.01)。结论大豆甙元对小鼠肝癌H22细胞的生长具有明显抑制作用,可诱导H22细胞凋亡,并在一定程度上抑制小鼠移植性肿瘤的生长,增强宿主免疫功能。
Objective To investigate the effect of daidzein on apoptosis of H22 tumor cells and its mechanism. Methods The effects of different dosages of daidzein (0.1-150.0 μmol / L) on the proliferation of H22 cells were detected by MTT assay. Sixty male Kunming mice were randomly divided into 6 groups (n = 10) The rats in model group and control group (serum-free RPMI 1640 medium), low, middle and high doses of daidzein (daidzein of 501, 00 and 200 mg / kg respectively) , And the positive control group (cyclophosphamide 30 mg / kg). The body weight, spleen index, thymus index and tumor weight of the mice in each group were compared 14 days after administration. The protein expressions of bax and bcl-2 were detected by western blot. Results The results of MTT assay showed that daidzein inhibited the proliferation of H22 cells, and the inhibition rate was 30.3% at the dosage of 150μmol / L daidzein for 72 h, which was significantly inhibited compared with the low dose group (P <0.05) Compared with the control group, the expression of bax increased by 50.05% and the expression of bcl-2 decreased by 52.62% in 10.0μmol / L daidzein group, while the expression of Bax and Bcl-2 decreased in medium and high dose of soybean The thymus index and spleen index in the aglycone group were significantly increased (P <0.01). Compared with the model group, the tumor weight index in the daidzein group decreased significantly (P <0.01). Conclusion Daidzein significantly inhibits the growth of mouse hepatoma H22 cells, induces the apoptosis of H22 cells and inhibits the growth of transplanted tumors in mice to a certain extent, and enhances the host immune function.