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[目的]探讨醇溶性蟾蜍毒素对H22荷瘤小鼠肝癌细胞凋亡的影响。[方法]用乙醇溶解蟾蜍分泌物原浆,采用减压蒸馏等方法进一步部分分离纯化,获得醇溶性的蟾蜍毒素混合物(EET),将其作用于H22荷瘤小鼠模型,计算肿瘤抑制率,应用光、电镜法观察H22肝癌细胞凋亡的变化。[结果]蟾蜍毒素组和氟脲嘧啶组的肿瘤抑制率分别是25.6%和30.8%(P<0.05),与对照组比较,肿瘤团块较小,细胞体积较小,异型性不明显,病理性核分裂较少,间质血管较少,坏死现象较轻。电镜下可见凋亡小体形成。[结论]蟾蜍毒素可通过诱导H22荷瘤小鼠肝癌细胞凋亡来达到抗肿瘤的作用。
[Objective] To explore the effect of alcohol-soluble scorpion toxin on the apoptosis of hepatoma cells in H22 tumor-bearing mice. [Methods] The scorpion secretions were solubilized with ethanol, and further fractionated and purified by vacuum distillation to obtain the alcohol-soluble scorpion toxin mixture (EET). The effect was evaluated in a H22 tumor-bearing mouse model and the tumor inhibition rate was calculated. Light and electron microscopy were used to observe the apoptosis of H22 hepatoma cells. [Results] The tumor inhibition rates of scorpion group and fluorouracil group were 25.6% and 30.8%, respectively (P<0.05). Compared with the control group, the tumor mass was smaller, the cell volume was smaller, and the heterotypicity was not obvious. Sexual karyolysis is less, interstitial vessels are less, and necrosis is lighter. Apoptotic bodies were observed under electron microscope. [Conclusion] Scorpionotoxin can achieve anti-tumor effect by inducing apoptosis of H22 tumor-bearing mouse hepatoma cells.