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目的通过不孕症夫妇标本中NUC-DNA定量结果分析,了解其患者集中的拷贝数和平均值并为临床药物治疗提供依据。方法采用FQ-PCR技术检测656份女性不孕症患者宫颈分泌物和789份男性不孕症患者精液标本中NUC-DNA。结果656份女性不孕症患者宫颈分泌物标本中CT-DNA阳性率为1.99%(12/603);UU-DNA阳性率为50.15%(329/656);NG-DNA阳性率为0.51%(3/591);789份男性不孕症患者精液中CT-DNA阳性率为1.10%(8/728);UU-DNA阳性率为13.56%(107/789);NG-DNA阳性率为0.52%(3/578)。结论西安地区女性不孕症患者宫颈分泌物中UU阳性率较男性高,男女CT、NG阳性率很低,逐渐下降趋势。了解不孕症夫妇标本中NUC-DNA定量结果对不孕症夫妇NUC感染的临床治疗有指导意义。
Objective To analyze the quantitative results of NUC-DNA in couples’ specimens of infertility, and to find out the copy number and average concentration in their patients and to provide basis for clinical drug therapy. Methods FQ-PCR was used to detect NUC-DNA in seminal fluid of 656 women with infertility and 789 men with infertility. Results The positive rate of CT-DNA in cervical secretions from 656 female infertility patients was 1.99% (12/603); the positive rate of UU-DNA was 50.15% (329/656); the positive rate of NG-DNA was 0.51% 3/591). The positive rate of CT-DNA in semen of 789 infertile men was 1.10% (8/728), the positive rate of UU-DNA was 13.56% (107/789), the positive rate of NG-DNA was 0.52% (3/578). Conclusion The positive rate of UU in cervical secretions of women with infertility in Xi’an is higher than that of men. The positive rates of CT and NG in men and women are very low and gradually decrease. It is instructive to understand the quantitative results of NUC-DNA in infertile couple specimens for clinical treatment of infertile couples with NUC infection.