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以等渗盐水抽提宫颈鳞状上皮癌21例及正常宫颈组织8例,制成水溶性膜旦白。其流程是剪碎组织、匀浆、冰冻、融解及超声波等使细胞充分破碎释放膜抗原,离心去沉渣得上清液等步骤供分析制备用。经分辨力高的聚丙烯酰胺凝胶电泳(T:9%,C:2%)分离:结果是肿瘤组织的旦白带有17—18条,正常组织的旦白带有15—16条。唯独在5、6位置的两条旦白带是正常宫颈组织从未出现过,可能是宫颈鳞状上皮癌的相关抗原:即一条为肿瘤抗原,一条为 HSV—2所致诱导抗原,但待用血清免疫学方法来证实。经等电聚焦凝胶电泳(T:6.6%,C:2.6%;2%两性载体电解质 pH4—10;上槽电泳液0.2%H_2SO_4,下槽电泳液0.2%NaOH)的分离:结果是肿瘤组织中的旦白含有出现在 pH6.25和 pH6.55的位置上的旦白,其他则两者是一致的,说明正常宫颈组织中缺少 pH6.25和 pH6.55位置的旦白质。由此结果提示:宫颈鳞状上皮癌的两个相关抗原的等电点,一为 pH6.25,一为 pH6.55。这个结果仅是初步,尚需更多病例来证实。
Twenty-one cases of squamous cell carcinoma of the cervix and eight cases of normal cervical tissue were extracted with isotonic saline to make water-soluble membrane protein. The procedure is to cut the tissue, homogenate, freeze, thaw, and ultrasound to fully break the cell to release the membrane antigen, to obtain the supernatant by centrifugation to obtain sediment for analysis. High-resolution polyacrylamide gel electrophoresis (T: 9%, C: 2%) was isolated: the result was a denatured tumor tissue with 17-18 bands and normal tissues with 15-16 bands. Only in the 5,6 position of the two Dan zone is normal cervical tissue has never been seen before, may be related to cervical squamous cell carcinoma antigen: a tumor antigen, one induced by HSV-2 antigen, but to be Serological immunological methods to confirm. Isoelectric focusing gel electrophoresis (T: 6.6%, C: 2.6%; 2% ampholyte electrolyte pH4-10; 0.2% H_2SO_4 in the upper tank electrophoresis solution, 0.2% NaOH in the lower tank electrophoresis solution): the result was tumor tissue The protein contained in the white at pH6.25 and pH6.55 position on the white, the other two are the same, indicating the lack of normal cervical tissue pH6.25 and pH6.55 position of the protein. The results suggest that: cervical squamous cell carcinoma of the two related antigen isoelectric point, a pH6.25, a pH6.55. This result is only preliminary, still need more cases to confirm.