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将小鼠IL-18基因和日本血吸虫Sj23膜蛋白基因分别插入pVAX1载体,构建真核表达质粒pVAX/ mIL-18和pVAX/Sj23 ,联合或单独免疫小鼠,以pVAX1空载体作对照,免疫2次,间隔2周,2次免疫后4周攻击日本血吸虫尾蚴。体液和细胞免疫检测结果表明,联合免疫组能诱导小鼠产生较强的抗日本血吸虫成虫可溶性抗原(SWAP)IgG,较高水平的IFN-γ和IL-2。攻虫试验表明,联合免疫小鼠成虫减虫率和肝脏减卵率分别达41 .6 %和49 .4 %,明显高于pVAX/Sj23单独免疫组(减虫率和肝脏减卵率分别为26 .5 %和41 .4 %)。以上试验结果表明,IL-18能明显增强Sj23 DNA疫苗在小鼠体内的免疫应答,并且产生较强的保护作用。
The mouse IL-18 gene and the Schistosoma japonicum Sj23 membrane protein gene were inserted into pVAX1 vector respectively to construct eukaryotic expression plasmids pVAX / mIL-18 and pVAX / Sj23. The mice were immunized with empty vector pVAX1 and immunized with empty vector 2 Times, 2 weeks interval, 4 weeks after 2 immunizations attack Schistosoma japonicum cercariae. The results of humoral and cellular immunity test showed that the combination immunization group can induce the mice to produce stronger IgG against SWAP and higher levels of IFN-γ and IL-2. The test of worm attack showed that the worm reduction rate and the egg reduction rate of the co-immunized mice were 41.6% and 49.4%, respectively, which were significantly higher than those of the pVAX / Sj23 immunized group (worm reduction rate and liver egg reduction rate were 26.5% and 41.4%). The above experimental results show that, IL-18 can significantly enhance the Sj23 DNA vaccine in mice immune response, and have a strong protective effect.