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在室内对转基因水稻KMD1中的Cry1Ab毒蛋白经食物链在几种主要害虫及其捕食性天敌体内的积累进行了研究。结果表明 :无论是水稻孕穗期还是成熟期 ,二化螟Chilosuppressalis连续取食KMD1或取食KMD136h后移至对照品种秀水 11上取食不同时间后 ,幼虫体内的Cry1Ab含量均随取食时间延长逐渐下降。稻眼蝶Mycalesisgotama幼虫连续取食KMD1或在KMD1上取食两天后移至秀水 11上继续取食不同时间 ,体内的Cry1Ab含量也都随取食时间延长而下降 ,但下降速度较二化螟更快。取食KMD1的二化螟和稻眼蝶幼虫的粪便中均检测到较高浓度的Cry1Ab ,对照组中均无Cry1Ab。取食KMD1的二化螟幼虫血淋巴中检测到Cry1Ab ,含量为 3 5ng g。取食KMD1的褐飞虱Nilaparvatalugens、稻蚜Sitobionavenae以及饲喂取食过KMD1的二化螟或稻眼蝶幼虫的拟水狼蛛Piratasubpiraticus体内都含有一定浓度的Cry1Ab ,其中 ,拟水狼蛛体内的CrylAb含量以饲喂取食KMD1稻眼蝶幼虫的含量最高 ,约为饲喂取食KMD1二化螟幼虫的 6 0倍。这些结果表明Cry1Ab可以沿水稻 害虫 天敌食物链传递
The accumulation of Cry1Ab toxin in transgenic rice KMD1 was studied indoors by several major pests and their predatory enemies via the food chain. The results showed that the content of Cry1Ab in larvae gradually increased with the prolongation of feeding time after either feeding on KMD1 or KMD136h and then on the control cultivar Xiushui 11 at both booting stages and maturing stages of Chilosuppressalis decline. After continuous feeding of KMD1 on rice larvae Mycalesisgotama larvae or on KMD1 for two days and then on Xiushui 11 for further feeding, the Cry1Ab content in the body decreased with the prolongation of feeding time, fast. Higher concentrations of Cry1Ab were detected in the stools of the rice stem borer and rice butterfly larvae fed KMD1, respectively, and none in the control group. Cry1Ab was detected in the haemolymph of the larvae of the rice stem borer, KMD1, in an amount of 35 ng g. Nilaparvatalugens, Sitobionavenae, and Sitobionavenae fed on KMD1 and Piratasubpiraticus fed on the rice stem borer (Chilo suppressalis or Rice Butterfly larvae) fed with KMD1 contained a certain concentration of Cry1Ab. The CrylAb content in the body To feed the feeding KMD1 rice butterfly larvae the highest content, about feeding KMD1 Stem borer larvae 60 times. These results indicate that Cry1Ab can be transmitted along the food chain of rice pest predators