[Objective] To research the inhibitory effects of black garlic extracting solution on the growth of colon cancer Ht-29 through PI3 K /AKT signal pathway. [Method] The tumor-bearing C57 BL /6 mouse model was established. Mice were given different concentrations of black garlic extracting solutions and PI3 K inhibitor LY294002. At the end of the test,the tumors were peeled off and weighed to calculate the inhibitory rate of tumor growth. Akt protein expression was detected by immunohistochemistry. Bad and PTEN protein expression was analyzed by Western blot. And the expression of Caspase-9 was detected by Realtime-PCR. [Results] The tumor inhibitory rate of black garlic extracting solution on mice colon cancer reached 49. 93%. Black garlic extracting solution restricted the expression of Akt and p Bad,but enhanced the expression of PTEN,Bad and caspase-9. [Conclusions] Black garlic extracting solution might restrict the growth of colon cancer Ht-29 through PI3K / AKT signal pathway.