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目的观察黄连素对兔颈动脉球囊损伤后一氧化氮(NO)、内皮素-1(ET-1)、血小板源生长因子(PDGF)及转化生长因子-β1(TGF-β1)水平的影响,并初步探讨其对内膜增生与血管重塑的作用。方法日本大耳白兔40只,随机分为假手术组、模型组、黄连素组和辛伐他汀组,除假手术组外各组均以球囊导管扩张损伤颈动脉内膜,并分别给予0.9%氯化钠溶液、黄连素注射液和辛伐他汀配制液各2.5mg.kg-1.d-1腹腔注射,假手术组仅普食喂养。术后15d检测血清NO、PDGF、TGF-β含量及血浆ET-1浓度,并取颈动脉切片作病理形态学观察。结果与模型组相比,术后15d黄连素组血清NO含量显著升高(P<0.01),而辛伐他汀组升高不明显;黄连素组与辛伐他汀组ET-1、PDGF和TGF-β1水平均显著低于模型组(均P<0.01)。结论黄连素可能通过升高兔颈动脉球囊损伤后NO浓度,降低ET-1及PDGF、TGF-β1水平,抑制内膜增生与血管重塑。
Objective To observe the effects of berberine on nitric oxide (NO), endothelin-1 (ET-1), platelet-derived growth factor (PDGF) and transforming growth factor-β1 (TGF-β1) levels in rabbits after carotid balloon injury. , And preliminary explore its role in intimal hyperplasia and vascular remodeling. Methods Forty Japanese big-eared rabbits were randomly divided into sham operation group, model group, berberine group and simvastatin group. Except for sham operation group, all groups were inflated with balloon catheter to injure the carotid artery intima and were given respectively. 0.9% sodium chloride solution, berberine injection and simvastatin preparations were intraperitoneally injected at 2.5 mg.kg-1.d-1, and the sham operation group was only fed with food. Serum NO, PDGF, TGF-β and plasma ET-1 levels were measured on the 15th day after operation. Carotid artery sections were taken for pathological observation. Results Compared with the model group, serum NO levels in the berberine group increased significantly at postoperative 15 days (P<0.01), while those in the simvastatin group did not increase significantly; ET-1, PDGF, and TGF in the berberine group and simvastatin group. -β1 levels were significantly lower than the model group (all P <0.01). Conclusion Berberine may increase the concentration of NO after balloon injury in rabbits, decrease the levels of ET-1, PDGF and TGF-β1, and inhibit the intimal hyperplasia and vascular remodeling.