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通过高脂肪喂食诱导的C57BL/6j小鼠动物模型研究苦瓜提取物对肥胖和胰岛抵抗的保护作用。小鼠喂养2周后成功建立肥胖小鼠模型,随机分为正常对照组,肥胖小鼠模型组和不同浓度的苦瓜提取物灌胃组,灌胃质量分数分别为13.5、27和54 g/kg。以10周为期,每天以同等体积流质灌胃两次,其中,正常对照组和模型组用w=0.5%羧甲基纤维素钠灌胃,药物组用不同浓度的苦瓜提取物灌胃。记录小鼠每日食物摄入量和每周体质量。同时测定内脏白色脂肪组织质量、血清甘油三酯(TG)、胆固醇(CHO)、低密度脂蛋白胆固醇(LDLC)、高密度脂蛋白胆固醇(HDL-C)、血糖和胰岛素水平,最后根据HOMA法计算胰岛素抵抗指数,HE染色确定附睾白色脂肪组织的脂肪细胞是否肥大。结果显示,54 g/kg苦瓜提取物组对小鼠摄食无影响,但小鼠体质量、附睾和内脏白色脂肪组织总质量减少,该剂量的苦瓜提取物显著抑制被提升的血清TG、LDL-C、葡萄糖、胰岛素浓度和胰岛素抵抗指数;而27 g/kg苦瓜提取物则显著降低TG浓度;13.5、27和54 g/kg苦瓜提取物分别显著升高HDL-C水平;苦瓜提取物能剂量依赖性地减轻高脂饮食诱导的脂肪细胞肥大。
To investigate the protective effect of Balsam pear extract on obesity and islet resistance by C57BL / 6j mouse model induced by high fat feeding. Mice were fed for 2 weeks and obese mice were successfully established and randomly divided into normal control group, obese mice model group and different concentrations of bitter melon extract intragastric group, the intragastric gavage were 13.5, 27 and 54 g / kg . The rats in the normal control group and the model group were orally administered with sodium carboxymethylcellulose w = 0.5% at a period of 10 weeks. The drug group was given gavage with different concentrations of bitter melon extract. Record mice daily food intake and body mass per week. The visceral white adipose tissue mass, serum triglyceride (TG), cholesterol (LD), high density lipoprotein cholesterol (HDL-C), blood glucose and insulin levels were measured at the same time. Insulin resistance index was calculated and HE staining was used to determine whether fat cells of epididymal white adipose tissue were hypertrophy. The results showed that the 54 g / kg bitter melon extract group had no effect on mice feeding, but the total weight of mice body weight, epididymis and visceral white adipose tissue decreased, the dose of bitter melon extract significantly inhibited elevated serum TG, LDL- C, glucose, insulin concentration and insulin resistance index; 27 g / kg bitter melon extract significantly reduced TG concentration; 13.5, 27 and 54 g / kg bitter melon extract significantly increased HDL-C levels; bitter melon extract can dose Relieves fat cell hypertrophy induced by high-fat diet.