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为探索强直性脊椎炎与ＨＬＡ－Ｂ２７关联机理，建立非同位素地高辛标记的ＰＣＲ／ＳＳＯ方法对中国上海地区６８例ＨＬＡ－Ｂ２７阳性的强直性脊椎炎病人和７例ＨＬＡ－Ｂ２７阳性正常对照进行了ＨＬＡ－Ｂ２７等位基因的检测，结果显示在病人和正常对照组中Ｂ＊２７０４都是最常见的等位基因，各占７６．８％和７１．４％，Ｂ＊２７０５亦是常见的等位基因，分别占２０．３％和２８．６％，两组间比较差异无显著性；在病人组还检出Ｂ＊２７０６和Ｂ＊２７０７各１例，提示此两等位基因可能也与ＡＳ关联。此结果为研究强直性脊椎炎与ＨＬＡ－Ｂ２７关联的机制提供了有用的资料。
To explore the relationship between ankylosing spondylitis and HLA-B27, a non-isotope-labeled digoxigenin-labeled PCR / SSO method was developed in 68 HLA-B27 positive ankylosing spondylitis patients and 7 HLA-B27 positive controls in Shanghai, China The HLA-B27 allele was tested and the results showed that B * 2704 was the most common allele in both patients and controls, accounting for 76.8% and 71.4%, respectively, and B * 2705 was also common Of the alleles, accounting for 20.3% and 28.6%, respectively, with no significant difference between the two groups; one case of each of B * 2706 and B * 2707 was also detected in the patient group, suggesting that the two alleles may be Also associated with the AS. This result provides useful information for studying the mechanism of association between ankylosing spondylitis and HLA-B27.