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目的研究K+通道阻断剂四乙胺(TEA)对胰岛β细胞凋亡的影响。方法以γ干扰素(IFNγ)+白细胞介素1β(IL1β)或链脲佐菌素(STZ)作用于小鼠胰岛细胞(NIT细胞),同时加入TEA,通过AnnexinV碘化丙啶(PI)、PI、Rhodamine123染色,采用流式细胞仪检测细胞凋亡与细胞膜电势;用MTT法检测细胞活性。结果IFNγ+IL1β或STZ可明显诱导NIT细胞凋亡,诱导组细胞存活率、凋亡率与未诱导组及TEA组差异均有统计学意义(均P<0.01);TEA能抑制诱导的NIT细胞凋亡(均P<0.01)。结论胰岛β细胞凋亡过程中可能也存在K+外流现象,K+通道在胰岛β细胞的凋亡中可能起着重要作用;TEA可抑制凋亡刺激剂诱导的胰岛β细胞凋亡,其效应可能与阻止K+外流有关。
Objective To investigate the effect of K + channel blocker tetraethylamine (TEA) on pancreatic β cell apoptosis. Methods Mouse islet cells (NIT cells) were treated with interferon gamma (IFNγ) + interleukin 1β (IL1β) or streptozotocin (STZ), TEA was added simultaneously, and AnnexinV propidium iodide (PI) PI, Rhodamine123 staining. Cell apoptosis and cell membrane potential were detected by flow cytometry. Cell viability was measured by MTT assay. Results IFNγ + IL1β or STZ significantly induced apoptosis of NIT cells. The survival rate of induced cells was significantly higher than that of untreated cells and TEA cells (all P <0.01). TEA inhibited the induction of NIT cells Apoptosis (all P <0.01). Conclusion K + efflux may exist in the process of islet β-cell apoptosis. K + channel may play an important role in the apoptosis of pancreatic β-cell. TEA can inhibit apoptosis induced by apoptosis stimulator, and its effect may be related to Prevent K + outflow related.