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建立了高效液相色谱法分析测定尿中皮质激素的方法,该法可同时测定尿中醛固酮、可的松及皮质醇的水平,最低检出限为01mg/L,线性范围05~20mg/L,回收率达95%。方法简便、灵敏度较高,可作为常规手段推广应用。
A method for the determination of urinary corticosteroids by HPLC was established. The method can be used for the simultaneous determination of aldosterone, cortisone and cortisol in urine with a minimum detection limit of 0.1 mg / L and a linear range of 0.5 ~ 20mg / L, recovery rate of 95%. The method is simple and has high sensitivity and can be popularized and applied as a conventional method.