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目的:探讨多菌灵对雄性大鼠睾丸发育和生精功能的影响及其作用机制。方法:40只清洁级未成年Wistar雄性大鼠随机分为4组(对照组,低、中、高剂量组,每组10只),低、中、高剂量组经口灌胃不同浓度的多菌灵溶液(分别为20、100、200mg/kg体重),对照组给予吐温80溶液,1次/d,连续80d。染毒结束后,立即取睾丸和附睾,观察其形态。测定各组大鼠体重及右侧睾丸和附睾重量;取左侧附睾尾测定精子活率和精子数量;采用HE染色法、原位末端标记法(TUNEL)及免疫组化SABC法观察大鼠睾丸组织的病理学变化、细胞凋亡和Bcl-2/Bax表达情况。结果:中、高剂量组大鼠睾丸和附睾均明显萎缩、右侧睾丸和附睾重量减轻,左侧附睾尾精子活率和精子数量均低于对照组(P<0.01);中、高剂量组睾丸组织病理学检查可见明显异常;随多菌灵染毒浓度的增加,各剂量组生精细胞凋亡率逐渐升高,Bcl-2表达下降,Bax表达升高,与对照组比较差异有统计学意义(P<0.05,P<0.01)。结论:多菌灵可影响雄性大鼠的睾丸发育和生精功能,可能与下调Bcl-2和上调Bax致细胞凋亡增加有关。
Objective: To investigate the effect of carbendazim on testicular development and spermatogenesis in male rats and its mechanism. Methods: Forty clean Wistar male rats were randomly divided into 4 groups (control group, low, medium and high dose group, 10 rats in each group). The rats in low, medium and high dose groups were orally administered with different concentrations Spirulina solution (20, 100, 200 mg / kg body weight, respectively). The control group was given Tween 80 solution once a day for 80 days. After the end of the exposure, testis and epididymis immediately to observe the morphology. The weight of the testis and the weight of the epididymis were measured in the rats in each group. The sperm motility and sperm count were measured by the left epididymis. HE staining, TUNEL and immunohistochemistry SABC were used to observe the testis Pathological changes, apoptosis and Bcl-2 / Bax expression. Results: The testis and epididymis were significantly atrophied, the right testis and epididymis weight were reduced, the sperm motility and sperm count of the left epididymis were both lower than those of the control group (P <0.01) Testis histopathology showed obvious abnormalities. With the increase of the concentration of carbendazim, the apoptosis rate of spermatogenic cells in each dose group gradually increased, the expression of Bcl-2 decreased and the expression of Bax increased compared with the control group Significance (P <0.05, P <0.01). Conclusion: Carbendazim can affect testicular development and spermatogenesis in male rats, which may be related to the decrease of Bcl-2 and up-regulation of Bax.