论文部分内容阅读
目的:利用焦虑动物模型研究桑叶抗焦虑作用及其物质基础。方法:将小鼠随机分为空白对照组(蒸馏水0.375 mL)、地西泮对照组(0.0023 g/kg)、桑叶组(4.5 g/kg)、真空处理桑叶组(4.5 g/kg),每组10只,雌雄各半,灌胃给药,1次/d,连续灌胃4 d后,依次采用自主活动仪、明暗箱、高架十字迷宫考察药物对小鼠的行为影响;高效液相色谱法(HPLC)检测行为学实验后小鼠脑神经递质含量。结果:与空白对照组比较,真空处理桑叶组可显著增加小鼠在明箱停留时间(P<0.05),并显著提高小鼠开臂滞留时间百分比(OT%)(P<0.05)。小鼠脑内神经递质检测显示,真空处理桑叶组、桑叶组均可显著降低小鼠脑组织5-羟色胺(5-HT)浓度(P<0.05),去甲肾上腺素(NE)、多巴胺(DA)浓度也有降低,但无统计学差异。结论:桑叶具有抗焦虑作用,且其物质基础可能为γ-氨基丁酸(GABA)。
OBJECTIVE: To study the anti-anxiety effect and its material basis of mulberry leaves using animal models of anxiety. Methods: The mice were randomly divided into blank control group (0.375 mL distilled water), diazepam control group (0.0023 g / kg), mulberry leaf group (4.5 g / kg) and vacuum treated mulberry leaf group (4.5 g / kg) , Each group of 10, male and female, gavage, 1 times / d, continuous gavage 4 d, followed by autopilot, light and dark box, elevated plus maze to investigate the behavior of drugs on mice; high performance liquid The content of neurotransmitters in mice after behavioral experiment was detected by HPLC. Results: Compared with the blank control group, the mulberry leaf group treated with vacuum could significantly increase the stay time in the light box (P <0.05) and significantly increase the percentage of open arm retention time (OT%) (P <0.05). Neurotransmitters in the brain of mice showed that the concentrations of 5-HT (5-HT), norepinephrine (NE) Dopamine (DA) concentration also decreased, but no significant difference. Conclusion: Mulberry leaves have anti-anxiety effects, and its material basis may be γ-aminobutyric acid (GABA).