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目的检测组织学正常的涎腺组织中5个抑癌基因的甲基化情况,为进行涎腺肿瘤的甲基化研究提供参照。方法甲基化特异性PCR(methylation-specific polymerase chain reaction,MSP)法分析60例组织学正常的涎腺组织中E-钙黏蛋白(cadherin),p16,RASSF1A,DAPK和MGMT基因启动子区的甲基化水平,并与前期研究中涎腺腺样囊性癌中的甲基化水平相比较,同时分析正常涎腺组织中E-cadherin(E-cad),p16,RASSF1A,DAPK和MGMT基因的甲基化与患者的性别、年龄及吸烟之间的关系。结果 13%(8/60)涎腺组织中发现存在甲基化,包括7%(4/60)E-cad,4%(2/60)p16,4%(2/60)RASSF1A,4%(2/60)DAPK,2%(1/60)MGMT。与之前腺样囊性癌中的结果比较,E-cad(P<0.01),p16(P<0.01),RASSF1A(P<0.01),DAPK(P<0.01)基因的甲基化在肿瘤组织及腺体组织中有明显差异。但涎腺组织中E-cad,p16,RASSF1A,DAPK和MGMT基因的甲基化与患者的性别、年龄及吸烟均无明显的相关性。结论正常的涎腺组织中E-cad,p16,RASSF1A,DAPK和MGMT基因的甲基化均为少发事件。
Objective To detect the methylation status of 5 tumor suppressor genes in salivary gland tissue with histological normality and provide references for the methylation of salivary gland tumors. Methods Methylation-specific polymerase chain reaction (MSP) was used to analyze the expressions of E-cadherin, p16, RASSF1A, DAPK and MGMT promoter in 60 histologically normal salivary glands Methylation level, and compared with the methylation level in salivary adenoid cystic carcinoma in the previous study. Meanwhile, the expression of E-cadherin, p16, RASSF1A, DAPK and MGMT genes in normal salivary gland tissues The relationship between methylation and patient’s gender, age and smoking. Results Methylation was found in salivary glands of 13% (8/60), including 7% (4/60) E-cad, 4% (2/60) p16, 4% (2/60) RASSF1A, 4% (2/60) DAPK, 2% (1/60) MGMT. Compared with the results of adenoid cystic carcinoma, methylation of E-cad (P <0.01), p16 (P <0.01), RASSF1A (P <0.01) There are significant differences in glandular tissue. However, methylation of E-cad, p16, RASSF1A, DAPK and MGMT in salivary glands had no significant correlation with patient’s sex, age and smoking. Conclusion Methylation of E-cad, p16, RASSF1A, DAPK and MGMT genes in normal salivary glands is a rare event.