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背景:荞麦种子提取物具有降糖、降脂、抗氧化等活性,荞麦花提取的有效成分是否也具有降糖、降脂、抗氧化等作用,尚需进一步的研究。目的:研究荞麦花总黄酮(totalflavonesofbuchwheatflower,TFBF)对实验性2型糖尿病高脂血症大鼠降血糖、血脂等作用,并探讨其降血糖和调节血脂的作用机制。设计:完全随机对照的实验研究。地点、材料和干预:本实验在华北煤炭医学院药理学教研室和华北煤炭医学院附属医院同位素科完成。实验选用SD大鼠60只,清洁级Ⅱ级,体质量(220±20)g,雌雄各半。大鼠被随机分为正常对照组、实验对照组、TFBF0.15,0.30,0.60g/kg剂量治疗组和阳性对照药HB-419组,每组10只。用链脲佐菌素和高脂饲料诱发大鼠2型糖尿病和高脂血症,以TFBF口服治疗12周,剂量分别为0.15,0.30,0.60g/kg。主要观察指标:TFBF对实验性2型糖尿病高脂血症大鼠血中空腹血糖、糖耐量、血清总胆固醇、三酰甘油、低密度脂蛋白胆固醇(lowdensitylipoproteincholesterol,LDL-C)、高密度脂蛋白胆固醇(highdensitylipoproteincholesterol,HDL-C)、动脉粥样硬化指数(atherosclerosisindex,AI)、胰岛素及肝细胞膜胰岛素受体(Insulinreceptors,INS-R)结合力的影响。结果:TFBF治疗组大鼠血中空腹血糖,三酰甘油,LDL-C犤TFBF0.15,0.30,0.6
Background: Buckwheat seed extract has hypoglycemic, lipid-lowering, antioxidant and other activities. The active ingredients extracted from buckwheat flower also have hypoglycemic, lipid-lowering and anti-oxidation effects. Further research is needed. Objective: To investigate the effects of total flavones of buckwheat flower flavonoids (TFBF) on hypoglycemic and blood lipids in experimental type 2 diabetic hyperlipidemia rats, and to explore the mechanism of hypoglycemia and regulation of blood lipids. Design: A completely randomized controlled experimental study. Location, Materials, and Interventions: This experiment was performed in the Department of Pharmacology, North China Coal Medical College and the Isotope Division of the Affiliated Hospital of North China Coal Medical College. The experiment used 60 SD rats, clean grade II grade, body weight (220 ± 20) g, male and female. The rats were randomly divided into normal control group, experimental control group, TFBF 0.15, 0.30, 0.60 g/kg dose treatment group and positive control drug HB-419 group, 10 mice in each group. Rats with type 2 diabetes mellitus and hyperlipidemia were induced with streptozotocin and high-fat diet and were treated with oral TFBF for 12 weeks. The doses were 0.15, 0.30, and 0.60 g/kg, respectively. MAIN OUTCOME MEASURES: Blood FBG, glucose tolerance, serum total cholesterol, triacylglycerol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein in TFBF of experimental type 2 diabetic hyperlipidemia rats The effects of high density lipoprotein cholesterol (HDL-C), atherosclerosis index (AI), insulin, and insulin receptor (INS-R) binding on hepatocyte membranes. RESULTS: In the TFBF treatment group, the blood glucose, triglyceride, LDL-C犤TFBF0.15, 0.30, 0.6