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目的 建立ret基因重排的分析方法 ,提示中国人甲状腺乳头状癌中是否存在原癌基因ret重排及重排类型。方法 应用RT PCR方法对 65例甲状腺乳头状癌石蜡切片标本进行ret基因重排检测 ,并对扩增结果进行测序鉴定。结果 在提取RNA成功的 38例甲状腺乳头状癌 (PTC)标本中 ,有 71 %发生ret重排 ,其中 1 0 5 %表达PTC1 ;5 3 %表达PTC3 ;5 3 %表达PTC4 ,以PTC1发生的频率较高。仅 1例表达PTC2与PTC3 PTC4混合型重排 ,中国人PTC可能与ret和H4及ELE1重排激活相关。结论 中国人PTC组织中存在 4种重排 ,且以PTC1较为常见。单一标本中有多种重排形式并存 ,PTC1 +3重排形式 1 0例、3例PTC1 +4、1例PTC2 +3 +4、1例PTC3 +4、4例PTC1 +3 +4,单一标本中多种重排形式并存的比例高达 50 % ,但与癌症的恶性程度、转移等无必然联系
Objective To establish a ret gene rearrangement method that suggests the presence of ret rearrangement and rearrangement of protooncogenes in Chinese thyroid papillary carcinoma. Methods Reverse transcriptase PCR (RT PCR) was performed on 65 cases of papillary thyroid carcinoma paraffin-embedded specimens and the results were sequenced. Results In 38 samples of thyroid papillary carcinoma (PTC) with successful RNA extraction, there were 71% ret rearrangements, of which 105% expressed PTC1, 53% expressed PTC3, and 53% expressed PTC4 as PTC1 Higher frequency. Only one case of PTC2 and PTC3 PTC4 mixed rearrangement, Chinese PTC may be related to ret and H4 and ELE1 rearrangement and activation. Conclusion There are 4 kinds of rearrangements in Chinese PTC tissues, and PTC1 is more common. A single specimen coexist in a variety of rearrangements, PTC1 +3 rearrangement of 10 cases, 3 cases of PTC1 +4, 1 cases of PTC2 +3 +4, 1 cases of PTC3 +4, 4 cases of PTC1 +3 +4, single Specimens in a variety of rearrangements in the form of coexistence of up to 50%, but with the malignancy of cancer, metastasis, etc. are not necessarily related